Základy prietokovej cytometrie v rastlinnej biológii. Praktická analýza obsahu DNA v bunkovom jadre.

E-book

Vladislav Kolarčik

Flow cytometry is a diagnostic and research technique that enables rapid analysis of cells, nuclei and organelles of plants and other organisms. In plant biology, it is most often used for DNA content analysis in a wide range of applications. It is the method of first choice and its importance is even increasing compared to competing technologies. It is unrivaled in speed, accuracy, repeatability and low cost. Flow cytometry has become a standard tool in laboratories focused on biological-ecological plant research.

This textbook provides information on the historical development of flow cytometry and its establishment in the analysis of the plant genome. It describes the procedure of sample analysis to determine the nuclear DNA content and presents the basic applications, such as determination of genome size, ploidy level and reproduction modes of plants and determination of endopolyploidy level in plant tissues. These college textbooks are intended for students, teachers and anyone interested in the use of flow cytometry in scientific work with plant cells.

Download e-book for free (pdf)

Quantity

978-80-574-0047-9

Data sheet

Method of publication:
E-book (pdf)
Author:
Vladislav Kolarčik Rok vydania: 2021
Document type:
Course Notes
Number of pages:
74
Available from:
22.11.2021
Year of publication:
2021
Edition:
1st edition
Publication language:
Slovak
Faculty:
Prírodovedecká fakulta
License:
CC BY NC ND (Uveďte autora - Nepoužívajte komerčne - Nespracovávajte)
- Free for download

16 other products in the same category:

Nanotechnology

Nanotechnology

€17.68
Availability: 58 In Stock

 Andrej Oriňak - Renáta Oriňaková - Andrea Fedorková

Nanotechnology represent the fastest developing science in the present. They are based on the technologies that enable the matter manipulation at atomic level. Nanotechnology offer prepartion of the new materials with unknown and very specific properties and functions.Recent nanotechnology still stay in formative phase of their development. But more and more they are visible with nano-specific phenomenons in the fundamental control of the properties as well mater behaviour. This is quite possible that without nanotechnology will not work any science to the future, moreover that science will not exist anymore. Nanotechnolgy will give a chance and force to transform world to more environmental and comfortable. Expected is expansion of the nanotechnolgy to molecular nanosystems – heterogenous nets, in which molecules and supramolecules work as the nanodevices. The computers and robots will be reduced to the small and ultrasmall dimensions. In medicine will foremost the application of nanotechnology in genetic therapy and elimination of ageing. Nanosurgery will work at molecular level, cancer cells will be identified early and filtered , then substituted with healthy and unchanged. It is necessary to inform university or high school students  about principles of nanotechnolgy, preparation of the nanostructures, functionality of them and to offer full representation about molecular systems and nanodevices; the methods of nanostructures characterization and about application in miniaturized systems. 

Authors

Geopriestorové technológie v bádateľsky orientovanom vyučovaní geografie

Geopriestorové technológie v bádateľsky...

E-book

E-book

Stela Csachová - Ján Kaňuk - Alena GessertLadislav NovotnýŠtefan GáborJozef Šupinský

This book addressed the inquiry-based learning and teaching with the support of geospatial technologies. Today, geospatial technologies are influencing geography teaching learning, particularly in secondary education. Theoretical framework gives the reader the insight to inquiry-based learning, with special focus on inquiry in geography. The one-by-one step of inquiry is analysed in detail. The major part of the book presents the eleven models of geography lessons, representing the subject content of physical, human and regional geographies, various levels of inquiry and use of different geospatial technologies. The readers are acquainted with the use of geographic information system (particularly Google Earth and ArcGIS online), web portals, webGIS, open data, mobile applications and new forms of 3D cartographic visualisation (virtual reality, 3D print, dynamic 3D map) in teaching geography. The lesson models were tested with the students to be geography teachers in school year 2021/2022. This books serves as resource mainly for undergraduate teacher students and in-service geography teachers.

Download e-book for free (pdf)

Otázky a úlohy zo všeobecnej chémie

Otázky a úlohy zo všeobecnej chémie

€15.00
Availability: 120 In Stock

Ivan Potočňák a kol.

The textbook represents a study literature on the subject of General chemistry, which is a compulsory subject of bachelor courses in Chemistry and Chemistry -  interdisciplinary study, the first year. This textbook contains 15 + 2 chapters in the whole range of 170 pages that follow the content of lectures and by means of examples and theoretical tasks consolidate and extend knowledge gained from lectures.

Praktikum z nukleárnej magnetickej rezonancie. Časť 2: Kvantitatívna analýza a fyzikálno-chemické štúdie

Praktikum z nukleárnej magnetickej rezonancie....

E-book

E-publication
Mária Vilková-Zuzana Kudličková-Ján Elečko
This study material is intended for students of chemistry and related disciplines interested in applied nuclear magnetic resonance (NMR) and physical chemistry. It offers practice-oriented tasks designed to develop skills in NMR spectroscopy, quantitative analysis, and data interpretation. Students learn modern approaches to determining physicochemical parameters (e.g., partition coefficients, pKa values, keto–enol equilibria, or enantiomeric excess) and gain experience with software tools for spectrum processing.
Download the e-book for free (pdf)

MAGNETICKÉ NANOČASTICE: NANOMAGNETIZMUS, PRÍPRAVA A POKROKOVÉ APLIKÁCIE

MAGNETICKÉ NANOČASTICE: NANOMAGNETIZMUS,...

E-book

Adriana Zeleňáková

Magnetic nanoparticles are extremely interesting magnetic objects that still hide much that is undiscovered, both because of their physical properties and because of their diverse applications. Iron nanoparticles, or iron oxides, are used in medical diagnostics for magnetic resonance imaging, in the treatment of hyperthermia, and an important application is the use of magnetic particles for targeted drug delivery.

The textbook "Magnetic Nanoparticles: Nanomagnetism, Preparation and Advanced Applications" is intended primarily for students of the Master's programme in Physics and students of related disciplines at universities and faculties with a physical, chemical, metallurgical and biophysical focus. The motivation for writing this textbook was to develop study material that would contribute to improving the quality of teaching in the profile subject Nanomaterials and Nanotechnology at the Institute of Physical, Faculty of Sciences, UPJŠ, in the Master's programme Condensed Matter Physics, as well as in the profile subject in the PhD programme Advanced Materials.

The university textbook is divided into eight chapters. The first and second chapters are devoted to basic information about magnetism, the origin of nanomagnetism in nanoparticles and surface phenomena. The third and fourth chapters are devoted to nanomagnetism in nanoparticle systems. The fifth chapter describes in detail individual protocols for investigating the phenomenon of superparamagnetism and the phenomenon of superspin glass using the SQUID device. The sixth chapter describes individual procedures for the preparation of magnetic nanoparticles and the seventh chapter describes individual experimental techniques for the study of magnetic nanoparticles. The final eighth chapter describes the applications of nanoparticles in biomedicine.

During the writing of this textbook, the chapters were prepared using the foreign-language specialist literature listed in the recommended reading list.

Download the e-book for free

Predictive Modeling of Soil Types and Their Characteristics

Predictive Modeling of Soil Types and Their...

E-book

E-book

Vasyl Cherlinka - Michal Gallay - Yuryi Dmytruk

The textbook is a practical guide to predictive mapping of soil types and properties in R. It bridges digital soil mapping theory with hands-on practice, covering the full workflow from data acquisition and preprocessing to validation and uncertainty quantification. Early chapters introduce the tidyverse ecosystem and the sf and terra packages for spatial data handling. Subsequent parts address modelling of categorical variables (soil types/WRB) and continuous variables (soil organic carbon) using Random Forest, Cubist, and Quantile Regression Forests. Step-by-step code examples show training/testing, feature selection, accuracy metrics, and model interpretation. Reproducibility and cartographic best practice are emphasised. A Slovakia case study demonstrates real-world application while outlining limits and generalisability. The book targets bachelor’s, master’s, and PhD students in geography, geoinformatics, environmental and agricultural sciences, as well as practitioners. Written in English, it fits international courses and mobility programmes.

Download the e-book for free (pdf)

Ecology of Soil Animals

Ecology of Soil Animals

E-book

E-book

Natália Raschmanová - Ľubomír Kováč

The text summarizes the basic knowledge about the functioning of the soil ecosystem with a focus on soil protists and dominant groups of soil animals. Characteristics of each soil animal group include basic morphology, ecology, distribution, population dynamics and their importance in the soil ecosystem. The aim of the text is to gain a basic understanding of the diversity and interactions of soil fauna with the soil environment. It deals with the ecological classification of soil fauna on the basis of body size, presence in the soil during the life cycle, food preference and by locomotion. It highlights important morphological and ecophysiological adaptations of soil fauna to physical and chemical environmental factors. The text also gives an overview of the basic methods in the field collection of soil fauna and their extraction from soil samples under laboratory conditions. It further informs on the method of taxonomic identification and ecological evaluation of soil fauna communities. Discusses the soil fauna of different types of ecosystems. It highlights the differences in soil fauna communities that inhabit grassland and forest habitats, and agricultural soils. This teaching material is intended for students in biology degree programmes.

Download the e-book for free (pdf)

Úvod do porovnávacej morfológie živočíchov

Úvod do porovnávacej morfológie živočíchov

E-book

E-book

Andrej Mock

The structure of the animal body is extremely varied. Each species has its distinctive morphological features, which bears as a testimony to long-term development and belonging to the developmental lineage. At the same time, the body has been tested for a long time by natural conditions, resulting in unique adaptations to the environment and life strategy. Despite this admirable variety - which includes both recent and fossil animals and which we have far from mapped – a comparison of body parts brings knowledge we can generalize: the main building principles, and boundaries within which phenotypic diversity is realized.

Comparative animal morphology today uses the latest methodological material and knowledge from other disciplines, from genetics, molecular biology and experimental embryology, through progressive imaging methods (e.g. electron microscopy, computer micro-tomography) and the possibilities of subsequent software processing of image material (measurement, staining, etc.) to the view of physics, chemistry, statics and geometry on the structures (to nanostructures) of the living body. Detailed knowledge of historical and contemporary conditions on Earth is an important interpretative context in the study of animal morphology. Surprisingly, the knowledge accumulated after centuries of research is now rapidly supplemented by new knowledge, and often the traditional knowledge is subsequently reinterpreted. Finally, and no less important, in studying animal (and human) morphology, we learn surprising knowledge about ourselves.

The author of the presented textbook had the ambition in a simplified form to introduce the reader, especially the student of biological studies, into a dynamic and fascinating scientific discipline, as the current comparative animal morphology is.

Stiahnite si e-knihu zadarmo (pdf)

Stresová fyziológia rastlín - Návody na cvičenia

Stresová fyziológia rastlín - Návody na cvičenia

E-book

E-book

Jozef Kováčik

These "Exercise Manuals" present a concise overview of commonly used methodologies as well as progressive techniques, such as the use of liquid chromatography and fluorescence microscopy. Individual tasks were selected based on the research focus of the Department of Botany at the Faculty of Science, Pavol Jozef Šafárik University in Košice, particularly in the context of "stress physiology."

Some tasks can also be utilized within the subject "Plant Ecology." Chamomile (Matricaria chamomilla) is typically used as a model plant for individual tasks due to its long-standing history of research at the Department of Botany and its ease of cultivation under laboratory conditions. The chapters also briefly discuss the significance of monitored parameters, principles of their determination, and metabolism (formation or degradation), aiming to indicate their mutual context.

This approach highlights that determining an individual parameter cannot provide a general explanation for the overall causes and impacts of observed changes; however, it can serve as a basis for testing hypotheses at the level of other parameters.

For deeper study of specific issues, there is an abundance of high-quality international monographs and review articles available.

Download the e-book for free (pdf)

Základné fyzikálne praktikum II.
  • On sale!
  • -€9.55

Základné fyzikálne praktikum II.

€10.55 €1.00 -€9.55
Availability: 21 In Stock

Peter Kollár a kol. 

Skriptá Základné fyzikálne praktikum II. sú určené pre študentov Prírodovedeckej fakulty UPJŠ v Košiciach študujúcich na študijných programoch fyzika a medziodborových programoch v kombinácii s fyzikou. Obsahom skrípt sú úlohy z elektriny, magnetizmu a optiky. Podľa študijných programov sú zaradené do 3.semestra bakalárskeho štúdia, v ktorom by už študenti mali mať absolvovaný predmet všeobecná fyzika II. (elektrina a magnetizmus). Súčasťou každej úlohy sú základné poznatky venované javom, ktoré sú obsahom samotnej úlohy.

Autori

Fotonika

Fotonika

E-book

E-book

Gregor Bánó

Photonics is an application-oriented discipline at the interface of optics, opto-electronics and quantum optics. It includes classical optics extended by the description of physical phenomena and optical devices that rely on quantum (photon) nature of light.
The main objective of the Photonics course is to provide a comprehensive overview of essential optical and photonic phenomena routinely applied in experimental scientific research while working with various light sources (e.g. in the field of biophotonics). The first chapters are devoted to the description of light at different levels. The logical order of subsections in this part corresponds to the structure of the textbook: B. E. A. Saleh, M. C. Teich, Fundamentals of Photonics. The following chapters contain a description of laser operation and an overview of selected optical instruments and their applications.
The body text is complemented by practical notes on the use of theoretical knowledge during daily work in the laboratory. The course of Photonics requires a basic knowledge of mathematics, electromagnetism and optics, and thus it is recommended to students who previously passed through the courses of the Bachelor's program in Physics.

Download the e-book for free (pdf)

Pokročilé elektrochemické metódy

Pokročilé elektrochemické metódy

E-book

E-book

Ivana Šišoláková - Radka Gorejová - Jana Shepa - Renáta Oriňaková

The university textbook "Advanced Electrochemical Methods" is a teaching aid intended for master's or doctoral students studying electrochemical subjects at the Department of Physical Chemistry, Faculty of Science, Pavol Jozef Šafárik University in Košice.

These textbooks follow and further develop textbooks focused on the basic electrochemical methods currently used in modern research. The presented teaching texts are processed in sixteen clear chapters, where they provide a theoretical principle and derivation of electrochemical processes and mechanisms in individual methods. Teaching texts also consist of practical laboratory exercises, in which students can try out the real application of the studied electrochemical methods.

The practical tasks are focused primarily on the demonstration of research that is carried out at our workplace. Students will be able to try, for example, electrochemical modification of the electrode surface, preparation of electrochemical sensors for the determination of analytes, preparation and characterization of a dye-sensitized solar cell or electrochemical evolution of hydrogen.

Download the e-book for free (pdf)

Vybrané kapitoly z elektrochémie

Vybrané kapitoly z elektrochémie

E-book

E-book

Kveta Markušová - Daniela Kladeková

Electrochemistry is the science that studies ionic systems and the processes or phenomena that occur at the interface between ionic systems and metals or semiconductors. At the current stage of development, electrochemistry can be divided into two main areas based on its focus. The first one - ionics - deals with the properties of ionic systems (solutions, melts, solid electrolytes). The second area - electrodeics - focuses on phenomena at the electrode/ionic system interface.

Both ionics and electrodeics investigate equilibrium and non-equilibrium phenomena and processes. Research into the properties of ionic systems under equilibrium conditions provides information and insights into the structure and composition of electrolyte solutions, melts, and solid electrolytes, while measurements under non-equilibrium conditions provide data on the electrical conductivity of ionic systems. In the field of electrodeics, the study of equilibria at the electrode/solution (or melt) interface is the domain of electrochemical thermodynamics. Measuring the rates of processes occurring at this phase interface and explaining the principles governing these processes fall under the scope of electrode process kinetics or electrochemical kinetics.

Download the e-book for free (pdf)

BIOLÓGIA LIŠAJNÍKOV. Časť C: Identifikácia metabolitov pomocou NMR spektroskopie

BIOLÓGIA LIŠAJNÍKOV. Časť C: Identifikácia...

E-book

E-book

Mária VilkováMonika GarberováMichal Goga

Nuclear Magnetic Resonance (NMR) is currently a widely used analytical tool that finds application in many fields of science and technology. The broadening of its practical use brings with it the necessity to create educational texts appropriate to the given scientific discipline.

This university textbook is intended for students of biology and serves as a supplement to the study materials for the advanced master's course "Biology of Lichens." At the same time, it represents a useful resource for master's and doctoral students involved in research, helping them interpret NMR spectra and present basic theoretical concepts.

The textbook briefly describes the important aspects of each NMR spectroscopic technique without delving excessively into the physical principles of individual methods or complex mathematical analyses. In this edition, the structures of lichen metabolites have been included, which students will isolate, purify, and identify using various analytical methods during practical exercises. An entire chapter is dedicated to a detailed description of solving the NMR spectra of isolated metabolites, with each step in elucidating the structures of both known and unknown metabolites explained in detail. The solved tasks are followed by numerous unsolved examples, including high-resolution 1D and 2D NMR spectra of metabolites, intended to further develop the acquired knowledge and skills of students and doctoral candidates in structure elucidation using NMR spectroscopy.

Download e-book for free (pdf)

Zoogeografia

Zoogeografia

€12.50
Availability: 10 In Stock

Ľubomír Kováč

Učebný text „Zoogeografia“ objasňuje základné princípy a historický vývoj rozšírenia živočíchov na Zemi, a ďalej analyzuje faktory, ktoré ho ovplyvňujú. Vďaka moderným vedeckým metódam a technikám výskumu je zoogeografia veľmi dynamicky sa rozvíjajúcou disciplínou. Z geologických etáp je podrobnejšie spracovaná problematika paleoklimatických cyklov kvartéru, holocénu a vývoja postglaciálnej klímy, ktoré zohrali významnú úlohu pri dynamike vývoja živočíšnych areálov. Areálová analýza charakterizuje a triedi areály živočíchov a ich dynamiku v nadväznosti na speciáciu. Podrobne je spracovaná problematika zoogeografických oblastí s dôrazom na palearktickú oblasť. Časť zameraná na dynamickú zoogeografiu rieši najmä problematiku refúgií a reliktov v súvislosti s vývojom pleistocénnej paleoklímy. Text stručnou formou sumarizuje základné antropogénne faktory, ktoré v recentnej dobe drastickým spôsobom limitujú rozšírenie živočíchov v rýchlo ubúdajúcom prírodnom prostredí na Zemi. Text ďalej prehľadným spôsobom charakterizuje biogeografiu Karpát a faunu územia Slovenska. Záverečná časť prezentuje fylogeografiu ako modernú vednú disciplínu zameranú na rekonštrukciu migračných ciest v postglaciáli, ktorá je podložená analýzou molekulárnych markerov populácií živočíchov v širšom geografickom kontexte. 

Autor

This website uses cookies to ensure you get the best experience on our website