Základy prietokovej cytometrie v rastlinnej biológii. Praktická analýza obsahu DNA v bunkovom jadre

Flow cytometry is a diagnostic and research technique that enables rapid analysis of cells, nuclei and organelles of plants and other organisms. In plant biology, it is most often used for DNA content analysis in a wide range of applications. It is the method of first choice and its importance is even increasing compared to competing technologies. It is unrivaled in speed, accuracy, repeatability and low cost. Flow cytometry has become a standard tool in laboratories focused on biological-ecological plant research. This textbook provides information on the historical development of flow cytometry and its establishment in the analysis of the plant genome. It describes the procedure of sample analysis to determine the nuclear DNA content and presents the basic applications, such as determination of genome size, ploidy level and reproduction modes of plants and determination of endopolyploidy level in plant tissues. These college textbooks are intended for students, teachers and anyone interested in the use of flow cytometry in scientific work with plant cells.